SimpleGeneKO^TM Kit (RNP method)

A RNP method KO all-in-one Kit SimpleGeneKO^TM Kit from SimpleGene Tech for human target gene knocking out, includes all reagents necessary for knocking out the target gene and sequencing primers for validating knockout efficiency. Achieves over 70% editing efficiency in cell lines and primary cells within just 3 days.

 

Advantage of SimpleGeneKO^TM Kit (RNP method)

1.From editing to verification, all-in-one box, making gene knockout accessible even for beginners.

 

2.Chemically synthesized sgRNA and efficient RNP delivery, with optimized sgRNA and Cas9 ratio.

 

3.A novel sgRNA design strategy targeting early exons.

 

4.Simple Sanger sequencing to determine knockout efficiency within just 3 days.

 

5.SimpleGene Tech has amassed extensive data demonstrating that this kit achieves high editing efficiency in cell lines, primary cells, iPS cells, T cells, etc.

 

Preparation Process

 

One SimpleGeneKO^TM Kit is sufficient for 10 reactions and includes: sgRNA, Cas9 protein, LM RNP transfection reagent, LM cell lysis solution, PCR primers for the target gene (upstream and downstream), and sequencing primers.