High-Throughput Gene Knockout Service
In 2020, the CRISPR/Cas9 technology was awarded the Nobel Prize. With the increasing maturity of gene editing techniques, they play an increasingly important role in numerous fields, including drug target discovery and verification, gene function research, and gene therapy, etc.
There is an urgent need in the global scientific research community for a series of target gene/whole-genome knockout cells to accelerate the progress of basic research, pharmaceutical research and development. Currently, more than a thousand articles have utilized the RNP gene knockout method, with its usage growing annually. This method is rapidly replacing the lentivirus and plasmid methods, becoming the preferred method for high-throughput gene knockout.
SimpleGene Tech boasts automated and high-throughput gene knockout standardization production platforms, capable of delivering hundreds of highly efficient KO cells every month. Our human whole-genome array knockout library has undergone rigorous high-content screening and validation, with over 80% of target genes achieving knockout efficiency of 70% or more.
High-Throughput Gene Knockout Workflow at SimpleGene Tech
Day 1-2: Evaluation
- Which cell should be chosen for gene knockout?
- Target gene expression identification in target cells: Deep proteomics analysis service.Day 2-6: Project Preparation
- Can it be knocked out?
- Necessity analysis of target genes in target cells: Essential Score analysis.Day 7: Cell Transfection
- How to knockout?
- SimpleGene Tech high-throughput gene knockout: RNP method.
Day 10: Knockout Efficiency Analysis
- How is the knockout efficiency?
- Target gene knockout efficiency analysis: Double guarantees by Sanger sequencing and proteomics analysis.
Why Choose SimpleGene Tech
1.Complete coverage of the human genome, targeting tens of thousands of genes, including thousands of drug development-related target genes.
2.For each target gene, three sgRNAs are designed to ensure high knockout (KO) efficiency.
3.The sgRNAs are specially chemically modified to improve stability and block innate immune responses.
4.The high KO efficiency has been experimentally verified, and a complete set of sequencing primers has been designed for the entire genome.
5.The editing efficiency can be determined in 3 days through simple Sanger sequencing.
Services at SimpleGene Tech
1.KO cell pool/cell line custom service.
2.High-Throughput solution for gene knockout library construction.
3.Drug target screening services based on high-throughput gene knockout array libraries.